After mixing well, 100 μl solution from this tube was transferred to the second MIC tube. One hundred μl stock solution was added in the first MIC tube containing 100 μl broth. Ten tubes, each having 100 μl thioglycollate broth were used for the MIC procedure. Minimum inhibitory concentration procedure lucidum has anti-microbial activity on P. The objective of this study was to evaluate whether or not spore powder of G. Researchers today are fast moving to tap the potential of medicinal plants and fungi with their antibacterial and antiviral activity. Research today focuses at discovering agents that specifically inhibit viral and bacterial multiplication without affecting the normal cells. This makes the development of new agents an urgent necessity. The mutation of the strains also complicates the issue. Most of the antibiotics and antivirals tend to exhibit undesired side effects and drug resistance. lucidum has demonstrated multiple functions such as blockade of release of histamine, inhibition of an overstimulated immune system, and a regulatory effect on cellular and humoral immunity. Scientific research has proved that spore powder of G. lucidum is widely used in Traditional Chinese Medicine. lucidum are polysaccharides, peptidoglycans, and triterpenes. The major physiologically active constituents in G. Various parts of this mushroom, namely the mycelia, spores, and fruit body possess medicinal properties. Ganoderma lucidum, a large, red mushroom, belonging to the class Basidiomycetes, is unique in its range of pharmacogenic components. Despite this, there are only a few published studies on this organism. intermedia, play an important role in the onset and subsequent development of the polymicrobial periodontal diseases. In the oral cavity, these bacteria, particularly P. Black-pigmented anaerobes have been considered as the key pathogens in mixed anaerobic infections. have been associated with chronic periodontitis. Among them, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Bacteroides spp., and Selenomonas spp. The bacteria comprises predominantly of Gram-negative anaerobic rods. The etiology of the disease is multifactorial and bacterial deposits play an essential role in the pathogenesis.
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